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Fig. 1 | Rice

Fig. 1

From: Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos

Fig. 1

Agrobacterium-mediated transformation of Oryza brachyantha W1711. a At 10 days after infection, GFP signals were observed in immature embryos. Bar = 1 mm. b Callus was cultured for 2 weeks on selection medium. Part of the callus showed a GFP signal. Bar = 2 mm. c After 2 weeks’ culture on regeneration media, shoots with strong GFP signals were formed. Bar = 3 mm. d Regenerated plants on rooting medium. Bar = 2 cm. e, f Leaves through e bright field and f GFP filters: left, transgenic; right, non-transgenic. Bar = 1 cm. g Transgenic plants growing to maturity without morphological abnormalities. Bar = 5 cm. h, i T1 seeds of a transgenic plant through h bright field and i GFP filters. T1 seeds with and without GFP signal were segregated. Bar = 4 mm

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