Genetic Dissection and Functional Differentiation of ALKa and ALKb, Two Natural Alleles of the ALK/SSIIa Gene, Responding to Low Gelatinization Temperature in Rice

Rice

Table 1 Gel permeation chromatography (GPC) parameters, relative crystallinities and infrared ratios (IR) among near-isogenic lines (NILs) ^a

Genotype	AP1 (%)	AP2 (%)	AP1/AP2	AM (%)	Relative crystallinity (%)	1047/1022 (cm⁻¹)
Nip(ALK^a)	63.15 ± 0.50a	23.47 ± 0.10a	2.69 ± 0.03b	13.37 ± 0.50a	31.7 ± 0.21b	0.389 ± 0.001b
NIL(ALK^b)	63.59 ± 1.20a	21.72 ± 0.50c	2.93 ± 0.07a	14.01 ± 0.70a	35.1 ± 0.14a	0.382 ± 0.002b
NIL(ALK^c)	64.18 ± 0.50a	22.56 ± 0.30b	2.85 ± 0.03a	13.27 ± 0.70a	35.9 ± 0.14a	0.397 ± 0.001a

^aData represent means ± standard deviations, n = 2. AP1, AP2, and AP1/AP2 represent the relative fractions correspond to short (AP1) and long (AP2) branch chains of amylopectin, and the degree of amylopectin branching (AP1/AP2), respectively. AM represents the true amylose content (AM) as determined by gel permeation chromatography (GPC) parameters. The relative crystallinity was calculated from XRD. Ratios of 1047/1022 represents the amount of ordered starch calculated from ATR-FTIR. The small letters in each column indicate significant differences among genotypes (P < 0.05)