Fig. 5
Resistance performance of transgenic OsHsfB4d-cas9 plants from the T2 generation. a Diagram of the Cas9 enzyme cleavage site and the genome editing of OsHsfB4d. Three mutation types, including a 1 base deletion, a 1 base insertion and a 4 bases deletion, were detected in the three transgenic lines. b Representative lesion sites from OsHsfB4d-cas9 plants at 14 dpi with Xoc strain RS105. c Lesion lengths in the OsHsfB4d-cas9 lines. Each line comprises seven individual transgenic plants assessed in this experiment at 6-week-old stage. “n.s.” means not significant according to the statistical analysis (n = 10). d Bacterial population growth in leaves of OsHsfB4d-cas9 and WT plants inoculated with RS105. The bars represent the mean ± SD. “n.s.”: not significant. e The transcript detection of OsHsp18.0-CI in 6-week-old wild-type and OsHsfB4d-cas9 lines. The bars represent the mean ± SD. f The phenotypes of the PXO99a lesions that developed on ZH11 and OsHsfB4d-cas9 lines at 14 dpi. g Lesion lengths were scored for OsHsfB4d-cas9 lines (n = 10). “n.s.”: not significant