Fig. 3
Resistance performance of transgenic OsHsfB4d OE plants from the T2 generation. a Diagram of the OsHsfB4d overexpression construct. b Relative expression levels of OsHsfB4d in three identified T2 transgenic lines. OsActin was used as an internal control. Bars represent the mean (three replicates for gene expression) ± SD. c Immunoblot analysis of OsHsfB4d in transgenic lines. Total protein extracts were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and probed with antibodies against the c-Myc tag (α-Myc). The stained Rubisco protein shows that equal amounts of protein sample were loaded. The immunoblot band shows OsHsfB4d-Myc of approximately 35 kD. d Representative lesion sites in OsHsfB4d OE leaves at 14 dpi with Xoc strain RS105. e Lesion lengths scored for T2 transgenic plants with pCXUN::OsHsfB4d. Eight individuals were scored for each line and the WT. “**” indicates significant (t test, P < 0.01) differences (n = 16). f Bacterial population growth in leaves from OsHsfB4d OE and WT plants inoculated with RS105. Colonies were counted with the leaf segment up to 5 cm from the inoculation site. Error bars represent the standard deviation of three independent leaves. The bars represent the mean ± SD. “**” indicates significant (t test, P < 0.01) differences. g The phenotypes of the PXO99a lesions that developed on ZH11 and OsHsfB4d-OE lines at 14 dpi. h Lesion lengths were scored for OsHsfB4d-OE lines (n = 10). Asterisks indicate significant difference between transgenic and wildtype (WT) plants at **P < 0.01 or *P < 0.05