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Fig. 2 | Rice

Fig. 2

From: OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae

Fig. 2

Fluorometric assays of GFP or LUC driven by OsHsp18.0-CI promoter constructs. a Diagram of the constructs of OsHsfB4d and the OsHsp18.0-CI promoter. b GFP fluorescence of the OsHsp18.0-CI promoter DNA construct (OsHsp18.0-CIPro:GFP). Coexpression with or without the OsHsfB4d in N. benthamiana. Images were photographed at 2 d after infiltration. Bar = 200 μm. c Quantitative analysis of the fluorescence intensity. The results are presented as the average fluorescence intensity using ZEN software. The experiments were repeated three times. ** indicates significant differences according to Fisher’s least-significant difference test (P < 0.01). d Luciferase assay of the co-expressed OsHsfB4d and the promoters of OsHsp18.0-CI (OsHsp18.0-CIPro:LUC). Mock represents the 10 mM MgCl2 solution

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