Fig. 7
Quantitative real-time PCR analysis of genes associated with chloroplast development and photosynthesis in the wild type and slc1 mutant leaves at the third-leaf stage. a Transcript analysis of chloroplast 23S, 16S, 5S and 4.5S rRNAs. b Transcript analysis of plastid-encoded and nucleus-encoded chloroplast development-related genes. c Transcript analysis of chlorophyll synthesis-related and photosynthesis-related genes. OsActin1 and OsUbiquitin were used as double internal controls, and relative transcript levels were analyzed using the 2-ΔΔCT method (Livak and Schmittgen 2001). Three biological replicates were performed. Error bars are SDs. The asterisks indicate statistical significance between the wild type and slc1 mutant, as determined by Student’s t-test (** P < 0.01)