Fig. 3

Map-based cloning of OsSLC1. a The OsSLC1 locus was mapped to chromosome 6 (Chr. 6). b The OsSLC1 locus was fine-mapped to a 50.7 kb interval on the BAC clone AP003634. c Open reading frame of the candidate gene Os06g0710800 in the mapped region; the mutation site is indicated by a black upright triangle. d T₀ mutant generated using the CRISPR/Cas9 system. e Independent T₁ complementation plants. WT, wild type. Scale bars, 2 cm