Fig. 5
From: Nitric Oxide Enhances Rice Resistance to Rice Black-Streaked Dwarf Virus Infection
NO-dependent protein S-nitrosylation. a Total protein was isolated from the mock- or RBSDV-inoculated Nipponbare or 15HPO187 plants. The isolated protein samples were analyzed by a modified biotin switch method followed by western blot assay using a biotin specific antibody. Lanes with darker labeling signal indicate stronger protein S-nitrosylation. b Total protein was isolated from the SNP-, old SNP- or cPTIO-treated and mock- or RBSDV-inoculated plants and analyzed as described in (a). Numbers on the left slide of the gels are the size of protein markers. c and d Images of gels stained with Coomassie Brilliant Blue, and used to show equal protein loadings. Images are representative of experiments repeated at least three separate times. The blot was quantified using ImageJ software (http://rsbweb.nih.gov/ij/). The data represented the means ± SD of the three replicates. M, protein marker. All the experiment were repeated 3 times and representative results are shown