Fig. 4

Changes in SA and defense responsive genes. a RBSDV disease incidence of the 500 μM SA- or 500 μM BTH pre-treated Nipponbare rice. 30 two-leaf stage rice seedlings of each cultivar were tests for disease incidence experiment. Images are representative of three independent biological experiments. The data represented the means ± SD of the three replicates. b to d Expressions of OsICS1, OsPR1b and OsWRKY 45 in the SNP-, old SNP- or cPTIO-treated RBSDV-inoculated plants determined by qRT-PCR. Expressions of OsUBC and OsActin1 in the rice plants were used as internal controls. The expression levels of OsICS1, OsPR1b and OsWRKY 45 in the water-treated RBSDV-inoculated plants were set as 1.0. Five plants were pooled together as one biological experiment. The data represented the means ± SD of the three biological replicates. Entire experiment repeated 3 times with similar results. Statistical differences between treatments in each time point were determined by the Duncan’s multiple test, p < 0.05