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Fig. 3 | Rice

Fig. 3

From: Nitric Oxide Enhances Rice Resistance to Rice Black-Streaked Dwarf Virus Infection

Fig. 3

Changes in Nipponbare rice resistance to RBSDV infection in response to NO-releasing reagents or NO scavenger. a RBSDV disease incidence of the 50 μM SNP or GSNO pre-treated rice. RBSDV inoculation was carried out using RBSDV viruliferous or non-viruliferous SBPHs. Results were from three independent experiments with 30 plants per treatment. The data represented the means ± SD of the three replicates. b RBSDV disease incidence of the 50 μM SNP or old SNP, or 100 μM cPTIO pre-treated Nipponbare rice. Plants pre-treated with water only were used as controls. RBSDV disease incidences were determined at 30 dpi. Results were from three independent experiments with 30 plants per treatment. The data represented the means ± SD of the three replicates. c NO production in the stems of the SNP-, old SNP- or cPTIO pre-treated mock- or RBSDV-inoculated Nipponbare plants. Stem sections with about 3 mm thick, were stained with DAF-FM DA, and examined and imaged under a confocal laser scanning microscope. Then, the signal representing NO production in stems was captured and analyzed using the ZEN software. Ten plants were analyzed as ten biological replicates per treatment. The data represented the means ± SD of the ten replicates. Entire experiment repeated 3 times with similar results. Different low case letters above the bars indicate the statistical differences between the treatments, by using the Duncan’s multiple test

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