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Rice
Fig. 5 | Rice

Fig. 5

From: An In Vivo Targeted Deletion of the Calmodulin-Binding Domain from Rice Glutamate Decarboxylase 3 (OsGAD3) Increases γ-Aminobutyric Acid Content in Grains

Fig. 5

In vitro enzyme assay for recombinant OsGADs based on the nucleotide sequence from genome-edited rice plants (#8_1 and #8_8). The GAD assay was performed to measure GAD production as in Fig. 1c. –Ca2+/CaM; without Ca2+/CaM, +Ca2+/CaM: with 0.5 mM Ca2+ and 0.1 μM bovine calmodulin (Sigma). Data present the mean ± standard deviation of three independent experiments for both

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