Fig. 3From: An In Vivo Targeted Deletion of the Calmodulin-Binding Domain from Rice Glutamate Decarboxylase 3 (OsGAD3) Increases γ-Aminobutyric Acid Content in GrainsDNA sequence of target regions in OsGAD3. Ni (wild-type variety Nipponbare) indicates nucleotide and amino acid sequences of the OsGAD3 gene in the target region of CRISPR/Cas9. Two black arrows (F1 and R1) indicate putative cleavage sites of CRISPR/Cas9. The CaMBD is indicated in blue. Double slash marks indicate the presence of sequence gaps. No. 1 to 8 indicate examples of mutations observed in the coding region of OsGAD3-CaMBD. Small letters in red indicate an insertion. Hyphens indicate a deletion. Base pairs (bp) in brackets indicate the length of the deletionBack to article page