TY - JOUR AU - Akama, Kazuhito AU - Akter, Nadia AU - Endo, Hinako AU - Kanesaki, Masako AU - Endo, Masaki AU - Toki, Seiichi PY - 2020 DA - 2020/03/16 TI - An In Vivo Targeted Deletion of the Calmodulin-Binding Domain from Rice Glutamate Decarboxylase 3 (OsGAD3) Increases γ-Aminobutyric Acid Content in Grains JO - Rice SP - 20 VL - 13 IS - 1 AB - Gamma-aminobutyric acid (GABA) is a non-protein amino acid present in all living things. GABA is mainly synthesized from glutamate by glutamate decarboxylase (GAD). In plants the enzymatic activity of GAD is activated by Ca2+/calmodulin binding (CaMBD) at the C-terminus in response to various stresses, allowing rapid GABA accumulation in cells. GABA plays a central role in not only stress responses but also many aspects of plant growth and development as a signaling molecules. Furthermore, it is known to be a health-promoting functional substance that exerts improvements in life-style related diseases such as hypertension, diabetes, hyperlipidemia, and so on. Previous reports indicated that CaMBD found plant GADs possess an autoinhibitory function because truncation of GAD resulted in extreme GABA accumulation in plant cells. Therefore, we attempted a genetic modification of rice GAD via genome editing technology to increase GABA levels in the edible part of rice. SN - 1939-8433 UR - https://doi.org/10.1186/s12284-020-00380-w DO - 10.1186/s12284-020-00380-w ID - Akama2020 ER -