Fig. 6

Depolymerization blocked the process of vacuole fusion. Aleurone layers were stripped from the intact seeds imbibed for 12 h, and then the isolated layers were incubated in a solution containing distilled water (control) alone or 10 μg/mL phalloidin (AF depolymerization inhibitor), 10 μg/mL cytochalasin B (CB) (AF depolymerizer), 10 μM Ac-YVAD-CMK (caspase-1 inhibitor) alone or in combination for 7 d, the layers were labeled with FITC-phalloidin, a the organization of AF (green) was observed by LSCM, and images were immediately captured. Scale bar is 10 μm. b Statistical analyses were conduced on the vacuole diameter per cell. Red cross represents the ruptured large central vacuole. Error bars indicate the means ± s.d., n = 3 biological independent experiments with at least three replicates for each. Bars with different alphabets show significant different at P<0.05 according to Duncan’s multiple range test