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Table 2 Summary of transformation frequencies and editing efficiency for five CRISPR-RNP/selectable marker plasmid co-delivery experiments

From: Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

Nuclease# embryos bombarded# hygR lines on regeration# putative lines to soilTransformation frequencya# lines analyzed by NGS# lines editedEditing efficiencyb
WT Cas960561016.7%5623.6%
HiFi Cas960341016.7%3438.8%
Cas9 D10A603046.7%3000.0%
AsCas12a603400.0%3400.0%
LbCas12a6042711.7%311032.3%
  1. aTransformation frequency = # putative lines to soil / # embryo bombarded × 100
  2. bMutation frequency = # mutated lines / # lines analyzed by NGS × 100