Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

Rice

Table 2 Summary of transformation frequencies and editing efficiency for five CRISPR-RNP/selectable marker plasmid co-delivery experiments

Nuclease	# embryos bombarded	# hygR lines on regeration	# putative lines to soil	Transformation frequency^a	# lines analyzed by NGS	# lines edited	Editing efficiency^b
WT Cas9	60	56	10	16.7%	56	2	3.6%
HiFi Cas9	60	34	10	16.7%	34	3	8.8%
Cas9 D10A	60	30	4	6.7%	30	0	0.0%
AsCas12a	60	34	0	0.0%	34	0	0.0%
LbCas12a	60	42	7	11.7%	31	10	32.3%

^aTransformation frequency = # putative lines to soil / # embryo bombarded × 100
^bMutation frequency = # mutated lines / # lines analyzed by NGS × 100