Table 1 crRNA and enzymes used in the experiment
Nuclease | crRNA ID | crRNA sequencea | PAM | GC % | Length (nt) |
|---|---|---|---|---|---|
WT Cas9 / HiFi Cas9 / Cas9 D10A | crRNA 1 | CGGGACAACTTCCTACTCAT | AGG | 50.0% | 20 |
Cas9 D10A | crRNA 2 | AGTTGCTTCAGCATGGATAC | TGG | 45.0% | 20 |
AsCas12a / LbCas12a | crRNA 3 | CGGGACAACTTCCTACTCATA | TTTG | 47.6% | 21 |