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Fig. 1 | Rice

Fig. 1

From: Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

Fig. 1

Choice of Carotenoid biosynthesis pathway to evaluate CRISPR-Cas nucleases. a Carotenoid biosynthesis pathway in rice. Phytoene desaturase (PDS) is a single copy gene involved in the synthesis of zeta-carotene from phytoene. GGPS, geranylgeranyl pyrophosphate synthase; PSY, phytoene synthase; PDS, phytoene desaturase; ZDS, zeta-carotene desaturase. b Schematic diagram showing OsPDS gene structure, OsPDS-Exon1 and relative position of crRNA1/2 for CRISPR-Cas9 and crRNA3 for CRISPR-Cas12a. PDS-F and PDS-R, forward and reverse primer pair for PCR and NGS analysis. c Flowchart showing RNP complex delivery and editing efficiency comparison analysis. d Regenerating green and albino rice plantlets on hygromycin containing rooting medium

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