Fig. 7
OsFPFL4 alters ROS accumulation in the root and inflorescence through affecting the expression of ROS-producer Rbohs. a Root system architecture of 7-day-old seedling in 1/2 MS medium. Scale bars, 1 cm. b NBT and DAB staining for O2− and H2O2, respectively, in the root tips of wild type and OsFPFL4 transgenic lines. Scale bars, 1 mm. c Quantification of H2O2 content in the roots of wild type and OsFPFL4 transgenic plants. Data are means ± SE (n = 10). d-kTranscript levels of OsRbohs in the roots of wild type and OsFPFL4 transgenic plants by qPCR analysis. Data are means ± SE (n = 3). Three replica experiments were performed. WT, wild type. OE-5 and OE-9, OsFPFL4 overexpression transgenic lines. RNAi-3 and RNAi-8, OsFPFL4-RNAi transgenic lines. Data are means ± SE (n = 10). The significant difference between OsFPFL4 transgenic lines and wild type was determined by Student’s t-test. *p < 0.05, **p < 0.01 or ***p < 0.001