Fig. 9
The flo14 mutant is defective in the splicing of mitochondria genes. Gene transcripts are labeled at the left. Spliced (S) and unspliced (U) transcripts are shown at the right. RNA were extracted from 12 days after flowering (DAF) endosperms of wild type (WT) and flo14 mutant (flo14). The splicing genes are tagged with red boxes. UBQ was used as a quantitative control. Nad 1–1: NADH dehydrogenase subunit 1–1, nad 1–2: NADH dehydrogenase subunit 1–2, nad 2: NADH dehydrogenase subunit 2, nad 2–1: NADH dehydrogenase subunit 2–1, nad 2–2: NADH dehydrogenase subunit 2–2, nad 3: NADH dehydrogenase subunit 3, nad 4–1: NADH dehydrogenase subunit 4–1, nad 4–2: NADH dehydrogenase subunit 4–2, nad 5: NADH dehydrogenase subunit 5, nad 6: NADH dehydrogenase subunit 6, nad 7: NADH dehydrogenase subunit 7, nad 9: NADH dehydrogenase subunit 9, atp 1: ATP synthase F0 subunit 1, atp 4: ATP synthase F0 subunit 4, cox1: cytochrome c oxidase subunit 1, cox 2: cytochrome c oxidase subunit II, cox 3–1: cytochrome c oxidase subunit 3–1, cox 3–2: cytochrome c oxidase subunit 3–2, ccmFc: cytochrome c biogenesis Fc (two pairs of primers were used), rpl 2: 50S ribosomal protein L2, rpl 5: ribosomal protein L5, rps 2: 30S ribosomal protein S2, rps 3: 30S ribosomal protein S3, rps 13: ribosomal protein S13, orf X: hypothetical protein, cyt b: cytochrome b