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Fig. 4 | Rice

Fig. 4

From: Lose-of-Function of a Rice Nucleolus-Localized Pentatricopeptide Repeat Protein Is Responsible for the floury endosperm14 Mutant Phenotypes

Fig. 4

Knockout of the OsNPPR3 gene by CRISPR system. a Seeds of the wild type (WT), flo14 mutant (flo14), and two independent CRISPR/Cas9 T1 transgenic lines, which were named CR9–1 and CR9–2. b Verification of the knockout lines by PCR-based sequencing. The representative transgenic lines were generated from Oryza sativa L. japonica variety Dianjingyou1 genetic background. ATG and TAG indicate the start and stop codons, respectively. UTR means untranslated region. Arabic number indicates the base position from the start codon. The target sequences designed for knocking out the Os03g0728200 by CRISPR/Cas9 system and the protospacer-adjacent motif (PAM) are underlined in blue and red, respectively. The missing bases are marked with red dotted lines. The chromatograms of the wild type and two knockout lines (CR9–1 and CR9–2) are shown. Triangle means the site of base deletion in the knockout line. Scale bars:1 mm in (a)

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