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Fig. 3 | Rice

Fig. 3

From: Lose-of-Function of a Rice Nucleolus-Localized Pentatricopeptide Repeat Protein Is Responsible for the floury endosperm14 Mutant Phenotypes

Fig. 3

Map-based cloning of the gene responsible for the OsNPPR3 phenotypes. a Fine mapping of the OsNPPR3 locus. The OsNPPR3 locus was mapped to a 165-Kb region by markers FY3–3 and FY3–6 on Chromosome 3 (Chr.3), which contains 15 predicted genes. The number of recombinants is indicated below the map. The candidate gene is indicated by red arrow. b The PPR gene structure and its protein. The lines indicate 5′-UTR and 3′-UTR, respectively. The blue box means exon. ATG and TAA represent start codon and stop codon. The PPR protein contains 10 PPR motifs. A single nucleotide substituted in the coding region of mutant gene leads to a premature stop codon. c Real-time RT-PCR analysis of OsNPPR3 in developing endosperms at 12 days after flowering (DAF) in the wild type (WT), flo14 mutant (flo14) and three flo14 mutant lines expressing the wild-type OsNPPR3 gene (Com1, Com2 and Com3). The value of Actin I mRNA was used as an internal control for data normalization. d Complementation of the flo14 mutant restored normal seed appearance. e Semi-thin sections of wild type and the complementation of the flo14 mutant (Com) endosperm at 15 days after flowering. Values are means ± SD (n = 3). The asterisks indicate statistical significance compared with the flo14 mutant, as determined by a Student’s t–test (** P < 0.01). Scale bars: 1 mm in (d), 100 μm in (e)

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