Fig. 6
From: OsIAGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Auxins in Rice
In vivo enzymatic activity of OsIAGT1 towards auxin. a Expression intensity of OsIAGT1 OE lines. Left, RT-PCR analysis of OsIAGT1 OE lines. Right, quantitative real-time PCR analysis of OsIAGT1 in OE-6 and OE-7. Values are means ±SD (n ≥ 3). Different lower-case letters indicate significant difference based on the Duncan’s multiple range test (p < 0.05). b HPLC analysis of endogenous IAA-Glc in wild type and OsIAGT1 OE lines. Left, no exogenous IAA was applied. Right, 100 μM IAA was applied for 12 h. 1 and 3 represent the presumable IAA-Glc; 2 and 4 represent the internal control (0.1 mM picloram) used in extraction process to monitor the recovery rate. c Relative content levels of endogenous IAA-Glc of wild type and OE lines. Left, no exogenous IAA was applied. Right, 100 μM IAA was applied for 12 h. Values are means ±SD (n ≥ 3). Different lower-case letters indicate significant difference based on the Duncan’s multiple range test (p < 0.05). d LC-MS/MS analysis of the endogenous IAA-Glc in rice. e Retention time of authentic standard IAA-Glc in HPLC analysis. f Ion peaks of authentic standard IAA-Glc in LC-MS/MS analysis under positive ion mode