Fig. 2From: How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysisDiagram of a plasmid construct used for multiplex genome editing. a Stacking of multiple sgRNA expression cassettes in one T-DNA. P Pol III: Polymerase III promoter. b Cloning of four tRNA-gRNAs into the CRISPR/Cas9 binary vector using a single Golden Gate ligation. (Modified from Kurata et al. 2018)Back to article page