Fig. 1

Phenotypes of the osgf14b mutant, complementation and OsGF14b-OE lines under drought stress treatment at the seedling stage. a Expression analysis of OsGF14b in the osgf14b mutant, complementation and OsGF14b-OE lines. The rice Actin1 gene was used as the internal control. Error bars represent the SE of three biological replicates. b The osgf14b mutant showed increased drought resistance. The 5.5- to 6.5-leaf stage seedlings of DJ, osgf14b and complementation lines (about 20 seedlings for each genotype) were subjected to drought stress without water for 12 d and then recovered for 7 d. The seedlings with newly growing leaf blades were counted as surviving plants and the survival rates were recorded. Error bars represent the SE of three biological replicates (**, P < 0.01, by Student’s t-test). c The OsGF14b-OE lines were more sensitive to drought stress treatment. The 5.5- to 6.5-leaf stage seedlings of Nip and OsGF14b-OE lines (about 10 seedlings for each genotype) were subjected to drought stress without water for 8 d and then recovered for 7 d. The seedlings with newly growing leaf blades were counted as surviving plants and the survival rates were recorded. Error bars represent the SE of three replicates (*, P < 0.05, by Student’s t-test). d-g The H₂O₂, MDA, proline and soluble sugar content in the WT and transgenic plants (mutant and OE) under normal growth and drought stress conditions. Error bars represent the SE of three biological replicates. Statistical differences are labeled with different letters according to the LSD test (P < 0.05, one-way ANOVA)