Table 2 Confirmation of Kbu peak sites and non-peak sites by quantitative PCR
Genomic region | Forward primer | Reverse primer | qPCR1 | qPCR2 | |
|---|---|---|---|---|---|
Peak sites | LOC_Os01g02960 | GACATGGTCACTGTCCCCAG | GATGCCATCTTCGTTGACGC | 0 | 1.23249 |
LOC_Os02g10180 | AATTACTTGCCACCGCCAGA | ATAGTCACCCTCCGCTTCCT | 0 | 1.475 | |
LOC_Os03g06210 | CGCGTGTACCGACGAGAAAA | TGTTGCCTACGTTCTCCACC | 0 | 1.807216 | |
LOC_Os04g37580 | TGCGTTGGGAATCAAACCCT | TCATCGTGGCTGGCTTATGG | 0 | 1.052905 | |
LOC_Os05g04950 | GGGGACATGTTGAGTGAGGG | CAACAACTGGCTGGGCAATC | 0 | 1.030004 | |
LOC_Os06g06410 | GAGCAAGGGCCCTAAGTTCG | TAGGCACTCACACATTCCGC | 0 | 0.794 | |
LOC_Os07g17220 | CAAAATTGCGAAGAACTGCCG | CCAGGCTCCCATATCCCTGAA | 0 | 0.568885 | |
LOC_Os08g08205 | GCCAGGTGAGATTAGGCCAG | TTCCTGACAAATGCCTGCCA | 0 | 1.656729 | |
LOC_Os09g28310 | TGTCCCACCCTAGAGACCAG | AGCTAGTCATCAGGCAGGTTG | 0 | 1.656729 | |
LOC_Os10g28254 | TCCGATTAGGTTGGCTATATTCAT | TGAAGCACTTCCACACAAGT | 0 | 0.382959 | |
LOC_Os11g26130 | GCCACTGTGTGAACCGACTA | AGGGTTGCCCTTGCGAATTA | 0 | 0.401704 | |
LOC_Os12g43750 | TTCCAAACCAACCAACTCCCT | TCCCACGAGAACATCACGGT | 0 | 1.184018 | |
Non-peak sites | LOC_Os01g01320 | TGGTGCACAATGCTGAGACT | CCAGTTTCAGAGTAGTTGATGGC | 0 | −1.34071 |
LOC_Os02g38870 | AGGAGGAAGAGGGGCCTAAG | TCGTGTCCATCTCCTCGTCT | 0 | −0.1424 | |
LOC_Os03g02650 | ATGGGCTTACGGGTGCATAG | CCTATCTCGCATACGTGCCG | 0 | 0.419453 | |
LOC_Os04g02030 | GAGCAAGGTCCTGGTCACAA | GGCCATCAAGACTCACAGCA | 0 | −0.32465 | |
LOC_Os05g25510 | TCTCAGTGGTGGGGAAGGAT | ACCATTGCTCACCTCAAGCA | 0 | −0.57654 | |
LOC_Os06g02930 | GGTCATGAAGGTCATCCACGG | CCGTGATGTTGGGCAAGTAGA | 0 | 1.961737 | |
LOC_Os07g37370 | GTGAGGGTGAGAGGGGAAAG | AAATTAGCTCCCGGACTGGC | 0 | 0.739465 | |
LOC_Os08g23640 | CAACGACATCGTGCTCGC | GCGACGCCGTACCTGAAG | 0 | −0.09366 | |
LOC_Os09g39170 | AACCCATCATCACGGTGGAC | AGAGATGGGCTGCTGGTAGA | 0 | 1.608844 | |
LOC_Os10g01590 | ATGACCACAAAACGGTTCGG | GATTGACTCGCGCTATGCAG | 0 | −0.97679 | |
LOC_Os11g02810 | GGATTTCGCGATGGGGATTC | AAGTGGTTCGCAACGCAATC | 0 | 0.866769 | |
LOC_Os12g43220 | GGTGCTAGGAATCGACCCAA | CTGCCATCACCAAGGGGAAT | 0 | −0.68791 |