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Fig. 3 | Rice

Fig. 3

From: Stress-Activated Protein Kinase OsSAPK9 Regulates Tolerance to Salt Stress and Resistance to Bacterial Blight in Rice

Fig. 3

OsSAPK9 interactions with OsSGT1 in vitro and in vivo. a OsSAPK9 interaction with OsSGT1 in yeast. AH109 yeast transformants diluted 10, 100, 1000, or 10,000 times were plated on synthetic dextrose (SD) medium lacking Trp and Leu amino acids (SD − L/T) or SD medium lacking Trp, Leu, His, and Ade amino acids (SD − L/T/W/A). b OsSAPK9 bound to OsSGT1 in the glutathione S-transferase (GST) pull-down assay. c OsSAPK9 interaction with OsSGT1 in Nicotiana benthamiana cells in the bimolecular fluorescence complementation assay. OsSAPK9 was fused to an N-terminal yellow fluorescent protein (NYFP-OsSAPK9) and OsSGT1 was fused to a C-terminal cyan fluorescent protein (CCFP-OsSGT1). d OsSAPK9 bound to OsSGT1 in the co-immunoprecipitation assay. Constructs encoding green fluorescent protein-tagged OsSAPK9 and Myc-tagged OsSGT1 were co-expressed in Agrobacterium-infiltrated N. benthamiana cells. Tissues were harvested 3 d after infiltration

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