Fig. 7
OsLEA3 and OsNCED3 are direct target genes of OsNAC2. The full-length OsNAC2 cDNA was fused to the GLA4 activation domain in the prey vector. The promoter sequence regions of different lengths were fused to an AbAi reporter gene in the bait vector. For yeast one-hybrid assay, Yeast cells were cotransformed with a bait vector and a prey vector and grown in liquid medium and diluted in a 10× dilution series (from 100 to 10− 3). Each dilution was spotted on both SD/−leu and SD/−leu with 300 ng/ml AbA to suppress the background and to test the strength of the interaction. L3, partial OsLEA3 promoter sequence; N6 and N5, partial OsNCED3 promoter sequences