Fig. 6

Pollen viability of the homozygous xa13 mutants. a Anthers in mature spikelets of Kitaake, homozygous mutant (T₃, 3A-6-1-4), and homozygous mutant (T₃, 4A-1-7-6). Scale bars, 1 mm. b Representative images of pollen viability tests from Kitaake and homozygous mutants (T₃, 3A-6-1-1 and 4A-1-7-1). Viable pollen grains are stained dark (gray arrow) and sterile pollen grains are stained light yellow (white arrow). Scale bars, 100 μm. c Statistical analysis of pollen viability of Kitaake, homozygous mutants (T₃, 3A-6-1-1 and 4A-1-7-1) lines. Pollen viability percentage was calculated relative to the total pollen counted in three microscopic images