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Fig. 3 | Rice

Fig. 3

From: CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Fig. 3

Generation of transgenic rice plants carrying the Cas9 transgene with a sgRNA targeting the Os8N3 gene. Genotyping was performed using the specific primers for Cas9, Cas9_RT_F and Cas9_RT_R (see Fig. 2b), from four independently transformed plants and their progenies (OsU6a xa13m/Kit T0, T1, T2, and T3 generations). Genomic DNAs were extracted from Kit (Kitaake) and OsU6a xa13m/Kit T0 (a), T1 (b), T2 (c), and T3 (d). ‘ × ’ indicates PCR negative

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