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Fig. 3 | Rice

Fig. 3

From: Overexpression of OsAGO1b Induces Adaxially Rolled Leaves by Affecting Leaf Abaxial Sclerenchymatous Cell Development in Rice

Fig. 3

Scheme of the OsAGO1b gene and characterization of the OsAGO1b protein. a Genomic organization of the OsAGO1b gene. The closed black boxes indicate exons, and connecting lines inidcate introns. The ATG start codon and TAA stop codon are also indicated. b Diagram of the OsAGO1b protein sequence and its main domains. The blue, green and red boxes indicate the Glycine-rice region, PAZ and Piwi domains, respectively. c Phylogenic analysis of AGO1 proteins from different plant species. The coding region sequences were aligned using Clustal W, and the evolutionary relationship was analyzed using the neighbor-joining method. Numbers on branches indicate the percentage of replicate trees in which the associated sequences clustered together in the bootstrap test (1000 replicates). The segment under the phylogenic tree is the evolutionary distance, which was computed using the Poisson correction method. The NCBI accesssion numbers for the proteins are presented in Additional file 3: Table S3. d Subcelluar localization of the YFP-OsAGO1b fusion protein in leaf sheath protoplasts isolated from 14-day-old seedlings of wild type Zhonghua 11 (ZH11). Scale bars = 10 μm. e Western blot analysis of OsAGO1b in nucleoprotein and cytoplasmic proteins isolated from 14-day-old ZH11 seedlings. T, total protein; N, nucleoprotein; C, cytoplasmic protein. Anti-OsAGO1b is the antibody of the OsAGO1b protein. Anti-H3 is the antibody of H3 protein. Anti-RNaseZ is the antibody of the RNaseZ protein

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