Fig. 2

Leaf segment-assay showing NaCl-induced senescence in rice leaves through analysis of chlorophyll and ROS (O₂⁻ and H₂O₂) content. a OsHBP1b over-expressing plant (L2 and L7) and WT plant leaf segments were kept in control (no NaCl) or different concentrations (100, 200 and 300 mM) of NaCl solution and pictures were taken after 72 h. The L2 and L7 leaves showing delayed leaf senescence as compared to the wild type leaves. b Chlorophyll contents (Chl-a, Chl-b and total Chl) in WT and transgenic rice leaf segments after 72 h of NaCl treatment. c Constitutive and salinity induced levels of O₂⁻ were measured by NBT staining in WT and. d Salinity- induced H₂O₂ content were measured by DAB staining in WT and OsHBP1b over-expressing plants. e H₂O₂ content in WT and OsHBP1b over-expressing plants measured through spectrophotometer. Error bars show standard error from triplicate experiments. Single asterisk show significant difference at p < 0.005 and double asterisk at p < 0.001