Table 1 Germination efficiency in T2-generation p35S-RGB1-Cas9 (Aa) and WT (Aa, RGB1 heterozygous mutants). (NG: Number of seeds, NGS: Number of germinated seeds, NNGS: Number of nongerminated seeds, GE: Germination efficiency)
From: The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice
WT | SG948–6 (Aa) | SG948–8 (Aa) | ||||
(a) | SG948–8-5 | SG948–6-9 | SG948–6-1 | SG948–6-11 | SG948–8-1 | SG948–8-8 |
NS | 40 | 40 | 40 | 40 | 40 | 40 |
NGS | 38.3 ± 0.6 | 38.7 ± 1.5 | 26.0 ± 2.6 | 23.7 ± 1.5 | 24.7 ± 3.5 | 23.3 ± 2.1 |
NNGS | 1.7 ± 0.6 | 1.3 ± 1.5 | 14 ± 2.6 | 16.3 ± 1.5 | 15.3 ± 3.5 | 16.7 ± 2.1 |
GE (%) | 95.83 ± 1.44 | 96.67 ± 3.82 | 65.0 ± 6.61** | 59.17 ± 3.82** | 61.67 ± 8.78** | 58.33 ± 5.20** |
Dehulled seeds were used. ** indicates a significant difference compared with the wild type at p < 0.01 | ||||||
WT | SG948–6 (Aa) | SG948–8 (Aa) | ||||
(b) | SG948–8-5 | SG948–6-9 | SG948–6-1 | SG948–6-11 | SG948–8-1 | SG948–8-8 |
NS | 96 | 96 | 96 | 96 | 96 | 96 |
NGS | 96.0 ± 0.0 | 96.0 ± 0.0 | 72.7 ± 2.5 | 69.7 ± 2.1 | 70.3 ± 3.1 | 69.7 ± 2.5 |
NNGS | 0.0 ± 0.0 | 0.0 ± 0.0 | 23.3 ± 2.5 | 26.3 ± 2.1 | 25.7 ± 3.1 | 26.3 ± 2.5 |
GE (%) | 100.0 ± 0 | 100.0 ± 0 | 75.69 ± 2.62** | 72.57 ± 2.17** | 73.26 ± 3.18** | 72.57 ± 2.62** |
Rice seeds were used. ** indicates a significant difference compared with the wild type at p < 0.01 | ||||||