Functional Identification of Novel Cell Death-inducing Effector Proteins from Magnaporthe oryzae

Rice

Table 1 Assays of growth, sporulation, and pathogenicity of the MoCDIPs overexpression and knock out mutants

Gene ID	Mutant	Vegetative growth (cm)	Sporulation (10⁴ spores·mL^− 1)	Pathogenicity
Gene ID	Mutant	Vegetative growth (cm)	Sporulation (10⁴ spores·mL^− 1)	Barley lesion (cm)^a	Rice seedling (Disease level)^b
	Guy11	6.59 ± 0.21	45.72 ± 6.48	6.71 ± 0.69	4–5
MGG_01532	OE-MoCDIP6	5.22 ± 0.08**	46.38 ± 7.36	6.25 ± 0.66	4–5
MGG_01532	Δ-MoCDIP6	6.60 ± 0.19	45.22 ± 8.50	7.05 ± 0.57	4–5
MGG_03354	OE-MoCDIP7	6.62 ± 0.14	46.60 ± 6.43	6.83 ± 0.58	4–5
MGG_03354	Δ-MoCDIP7	6.48 ± 0.17	39.22 ± 5.37	7.10 ± 0.70	4–5
MGG_05038	OE-MoCDIP8	6.59 ± 0.28	43.33 ± 4.97	6.13 ± 0.80	4–5
MGG_05038	Δ-MoCDIP8	6.83 ± 0.15	49.53 ± 6.62	6.42 ± 0.42	4–5
MGG_08411	OE-MoCDIP9	6.40 ± 0.07	47.23 ± 8.73	6.50 ± 0.48	4–5
MGG_08411	ND	ND	ND	ND	ND
MGG_12275	OE-MoCDIP10	6.55 ± 0.47	48.97 ± 6.89	6.88 ± 0.31	4–5
MGG_12275	Δ-MoCDIP10	6.65 ± 0.18	53.47 ± 7.80	6.63 ± 0.48	4–5
MGG_12521	OE-MoCDIP11	6.53 ± 0.19	43.10 ± 5.13	6.75 ± 0.50	4–5
MGG_12521	Δ-MoCDIP11	6.66 ± 0.13	51.58 ± 6.21	6.58 ± 0.30	4–5
MGG_13283	OE-MoCDIP12	6.51 ± 0.23	40.27 ± 6.90	6.21 ± 0.72	4–5
MGG_13283	ND	ND	ND	ND	ND
MGG_14371	OE-MoCDIP13	6.58 ± 0.09	39.72 ± 5.61	7.05 ± 0.50	4–5
MGG_14371	ND	ND	ND	ND	ND

^aInfection on detached barley leaves was assessed by measuring the diameter of the lesions on the leaves;
^bDisease severity was scored by following 0–5 scale (0–1: Resistant, 2: moderately resistant, 3: moderately susceptible, 4–5: severe susceptible);
Statistical significances (*P < 0.05 or **P < 0.01) between the MoCDIPs mutants and the wild type Guy11 were analyzed by student’s t-test; ND Not determined