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Table 1 Quality Control assessment of 21 trait-SNPs contain in the 1K-RiCA assay. The QC parameters ‘Utility’, ‘False Positive Rate’ (FPR), ‘False Negative Rate’ (FNR) were estimated for each SNP and haplotypes when more than 1 SNP was associated with a trait

From: 1k-RiCA (1K-Rice Custom Amplicon) a novel genotyping amplicon-based SNP assay for genetics and breeding applications in rice

SNP ID

Chr.

MSU7

Pos.

Gene

Negative Allele

Positive

Allele

Negative

Trait

Positive

Trait

Use as

SNP Utility

SNP FPR

SNP FNR

Haplotype Utility

Haplotype FPR

Haplotype FNR

chr03:16733441

3

16.73

GS3

G

T

Short

Long

Use as single SNP (T)

34.8%

0.0%

0.0%

   

chr05:437499

5

0.44

xa5

T

A

Susceptible

Resistant

Use as single SNP (A)

91.4%

0.0%

0.0%

   

chr06:27275515

6

27.28

Xa7

G

A

Susceptible

Resistant

Use haplotype (AAC)

 

96.2%

0.0%

0.0%

chr06:27627615

6

27.63

Xa7

C

A

chr06:27761109

6

27.76

Xa7

A

C

chr08:26448560

8

26.45

xa13

T

G

Susceptible

Resistant

Use haplotype (GGC)

 

81.8%

0.0%

0.0%

chr08:26709228

8

26.71

xa13

A

G

chr08:26898822

8

26.90

xa13

T

C

chr11:27603799

11

27.60

Xa4

C

A

Susceptible

Resistant

Use as single SNP (A)

52.4%

0.0%

0.0%

   

chr11:21190115

11

21.19

Xa21

C

T

Susceptible

Resistant

Use as single SNP (T)

98.4%

0.0%

0.0%

   

chr11:22162729

11

22.16

Xa23

C

T

Susceptible

Resistant

Use as haplotype (TTA)

 

96.5%

0.0%

0.0%

chr11:22453819

11

22.45

Xa23

C

T

chr11:23231455

11

23.23

Xa23

G

A

chr07:22119347

7

22.12

rstv

A

G

Susceptible

Resistant

Use as single SNP (G)

91.9%

0.0%

80.0%

   

chr09:5922125

9

5.92

sub1

C

T

Susceptible

Tolerant

Use haplotype (TAG)

 

88.1%

0.0%

0.0%

chr09:6252407

9

6.25

sub1

G

A

chr09:6913547

9

6.91

sub1

C

G

chr06:6752756

6

6.75

ALK

A

G

Low-GT

High-GT

Use as single SNP

98.8%

0.0%

60.0%

   

chr06:1765761

6

1.766

Wxt/wx

G

T

Depends on market preference

Depends on market preference

Use haplotype to differentiate high (GAC/GAC), intermediate (GCC) and low (TAC/TCC) ACC

84.3%

0.0%

0.0%

 

chr06:1768006

6

1.768

Wxin

(Wxg1)

A

C

60.8%

0.0%

75.0%

chr06:1768998

6

1.769

Wxa

(Wxg2)

C

T

75.2%

66.7%

100.0%