Fig. 4
From: Whole-Genome Sequencing Identifies a Rice Grain Shape Mutant, gs9–1
Gene and protein structures, and the relative expression of GS9–1. a The genomic and amino acid changes of line gs9–1. BC1F2-W is the wild-type pool, and BC1F2-M is the gs9–1 mutant pool that carries a 3-base (ATC) deletion. The amino acid (AA) sequence alignment shows that amino acid lysine (K) in Kitaake is substituted by amino acids asparagine (N) and glutamine (Q) in gs9–1. b qRT-PCR assays of LOC_Os09g02650 in Kitaake and gs9–1 in roots and the 2nd leaf sheath at seeding stage, the lateral bud at tillering stage and the young panicle at the fifth stage of panicle differentiation. The unpaired Student’s t-test showed that there is no different expression level between Kitaake and gs9–1. c Gene structure of LOC_Os09g02650 and the mutated sites of different alleles. d Conserved protein domains in LOC_Os09g02650 and protein structures of different mutant alleles. Motor domain, kinesin motor domain; SMC_N, the N terminal domain of the structural maintenance of chromosomes (SMC) proteins; Leu zipper, Leucine residues conserved in the bZIP protein; Neuromodulin-N, Gap junction protein N-terminal region. Information on putative conserved domains is retrieved from NCBI. ‘AA’ indicates amino acids. The asterisk indicates a premature translation termination. e The two mutated amino acids are indicated by black triangles in gs9–1. The height of a letter indicates this amino acid’s relative frequency at the given position (x-axis) predicated using the MEME program