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Rice
Fig. 5 | Rice

Fig. 5

From: Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice

Fig. 5

OsPP2C50 and OsPP2C53 physically interact with SAPK10 and their overexpression promotes water loss. a BiFC analysis of VN-SAPK10 and OsPP2C53-VC or OsPP2C50-VC. OsSar1p-VC was used as a negative control. Scale bars are 10 μm. b Co-IP analysis using OsPP2C53-GFP or OsPP2C50-GFP and SAPK10-Flag expressed in rice protoplasts. These experiments were repeated three times and a representative result was presented. c Water loss assays of OsPP2C53-overexpressing transgenic lines (E25–1, 2 and 3) and control line (A8: empty vector transgenic plant). d Water loss assay of OsPP2C50-overexpressing transgenic lines (E40–1, 4 and 5) and control line (A8: empty vector transgenic plant). The values depict the average and ± SD with 3 replications. ANOVA analyses were performed by comparing with water loss of A8 plant.

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