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Rice
Fig. 4 | Rice

Fig. 4

From: Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice

Fig. 4

Expression of OsPP2C50 and OsPP2C53 in rice guard cells and subcellular localizations of OsPP2CAs. a Subcellular localizations of nine OsPP2CAs were observed in rice protoplasts. Scale Bars = 10 μm. a Subcellular localization of OsPP2CA-GFPs in rice protoplasts. b, c, d Histochemical β-glucuronidase analysis of OsPP2CA promoter-GUS transgenic rice. Leaf surface of transgenic rice harboring OsPP2C06 promoter-GUS (D4–6), OsPP2C53 promoter-GUS (E48–1), and OsPP2C50 promoter-GUS (E46–2) with or without ABA treatments were stained with X-Gluc. Scale bars are 50 μm. e, f, g RT-qPCR analysis of OsPP2C06, OsPP2C50 and OsPP2C53 using two-week-old seedlings. The generated RQ (relative quantity, 2-ΔCt) was compared to the value of expression on shoot. OsUbi5 was used as endogenous control. The values depict the average and ± SD with 3 replications. h RT-qPCR analysis of OsPP2C06, OsPP2C50 and OsPP2C53 using two-week-old seedlings treated or not treated with ABA. The detected RQ (relative quantity, 2-ΔCt) displayed OsUbi5 was used as endogenous control. The values depict average and ± SD with 3 replications

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