Fig. 2
SAPK10 is expressed dominantly in rice guard cells and OsSAPK10-overexpressing transgenic rice presents less water loss. a Gene expression profiles of ten SAPKs in different rice cell types were presented as a heat map by using transcriptome analysis data from rice cells, black to yellow displayed increasing, black to blue displayed decreasing. b RT-qPCR analysis of OsSLAC1 gene expression in two-week-old seedlings. The generated RQ (relative quantity, 2-ΔCt) was compared to the value of expression on shoot. OsUbiqutin5 was used as endogenous control. The values depict the average and ± SD with 3 replications. c RT-pPCR analysis of OsSLAC1 gene expression in different tissues of mature rice (80 days old). The values were compared to the expression level on leaf blade. d Histochemical β-glucuronidase analysis of SAPK10 promoter-GUS transgenic rice. Left image is a 10-day-old seedling. Sh (sheath), Ti (tiller), Fl (flower), Cu (culm) and leaf blade epidermis. Scale bars are 50 μm. e A section of paraffin-embedded leaf blade stained with X-Gluc and eocin. Yellow arrows indicate guard cells. Scale bars are 50 μm. f Water loss assays of SAPK10-overexpressing transgenic lines (C7–5, 9 and 14) and control line (A8: empty vector transgenic rice). The picks depict the average and ± SD with 3 replications. ANOVA analysis was performed by comparing with water loss of A8 plant