Fig. 1
OsSLAC1 is specifically expressed in rice guard cells and OsSLAC1-GFP localizes to the plasma membrane in rice protoplasts. a RT-qPCR analysis of OsSLAC1 gene expression in two-week-old seedlings. The generated RQ (relative quantity, 2-ΔCt) was compared to the value of expression on shoot. OsUbi5 was used as endogenous control. The values depict the average and ± SD with 3 replications. b RT-qPCR analysis of OsSLAC1 gene expression in different tissues of mature rice (80 days old). The values were compared to the expression level on leaf blade. c Histochemical β-glucuronidase analysis of OsSLAC1 promoter-GUS transgenic rice. Left image is a 10-day-old seedling. Sh (sheath), Ti (tiller), Fl (flower), Cu (culm) and leaf blade epidermis (bottom right). Scale bars are 50 μm. d A section of paraffin-embedded leaf blade stained with X-Gluc (blue) and eocin. Yellow arrows indicate guard cells. Scale bars are 50 μm. e Subcellular localization of OsSLAC1-GFP and marker protein in rice protoplast. PM-mCherry is a plasma membrane localization marker. The scatterplot shows the values of fluorescence of the pixels across the two channels. Scale bars are 10 μm. f Water loss assays of OsSALC1-overexpressing transgenic lines (B76–1, 2 and 15) and control line (A8: empty vector transgenic plant). The values depict the average and ± SD with 3 replications. ANOVA analysis was performed by comparing with water loss of A8 plant