Fig. 3

Molecular identification of transformants. a PCR analysis of T₀ transformants shows the presence of the 1761 bp PTB1 gene in the rice genome (CK-), 412 bp fragment of PTB1 CDS in the pFS4 plasmid (CK+), and both 1761 bp and 412 bp fragments in T₀ transgenic plants. b Southern blot analysis to confirm insertion of T-DNA in transformants. Genomic DNA from a non-transgenic plant (CK-), the pFS4 plasmid (CK+), and T₀ generation transformants (1–7) were digested with EcoRI and hybridized with the PTB1 CDS probe. Southern blots show that PTB1 CDS sequence was stably integrated into the genomes of transgenic lines