Fig. 2

The increased susceptibility of oseds1 mutant to X. oryzae was associated with insertion of T-DNA. Two-tail student’s t-test. The “a” and “b” above bar indicate significant differences between wild-type (WT) and oseds1 plants at P < 0.01 and P < 0.05, respectively. a T-DNA insertion site in oseds1 mutant. T-DNA was inserted at position 587 of OsEDS1, counting the first nucleotide of translation start codon (ATG) as 1. TAA, translation stop codon. Arrows were PCR primers used for examination of the mutant. F9 + R, OsEDS1 primers; LSP2 + R, T-DNA, and OsEDS1 primers. b Homozygous oseds1 mutant was more susceptible to Xoo than heterozygotes and WT siblings (WT1). Data represent mean ± SE (n = 4 to 5). Plants 4, 6, 7 and 16 were homozygous oseds1 mutants, 1, 2, 5, 10, 11, 13, 14 and 15 were heterozygotes, and 3, 8, 9, and 12 were WT1. c Bacterial growth was analyzed at 12 days and disease symptoms at 14 days after Xoo infection. Data represent mean ± SE (n = 3). d oseds1 was more susceptible to Xoc. Lesion length and disease symptom were analyzed at seven days after Xoc infection. Data represent mean ± SE (n = 10 to 15). e oseds1 had a similar level of susceptibility to M. oryzae to that of WT plants. Disease index and disease symptom were analyzed at seven days after X. oryzae infection. Data represent mean ± SE (n = 23 to 28). f oseds1 affected a set of defense-related genes expression. Data represent mean ± SE (n = 3)