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Fig. 1 | Rice

Fig. 1

From: Jasmonic Acid-Involved OsEDS1 Signaling in Rice-Bacteria Interactions

Fig. 1

OsPAD4 directly interacted with OsEDS1. a OsPAD4 interacts with OsEDS1. BiFC analysis of OsEDS1 and OsPAD4 interaction. Fluorescence can be observed only in tobacco (Nicotiana benthamiana) cells co-transfected with OsPAD4-nYFP and OsEDS1-cYFP plasmids. Bars indicate 50 μm. b Protein pull-down assay for detection of His-TF-OsPAD4 and MBP-OsEDS1 interaction. Ten micrograms of His-TF-OsPAD4 coupled beads were used to pull down 5 μg MBP or MBP-OsEDS1 protein. Anti-MBP antibody was used to detect output protein. Anti-His antibody was used to detect input protein

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