Fig. 8

Pollen tube germination and growth of the WT and lssr1 lines in vivo. a Aniline blue staining of the WT pistils at 5–30 min after flowering (MAF). b Amplified pollen tube of the WT. c-e Little or no germinated pollen grains were detected on the lssr1 stigma at 5–30 MAF. f Multiple pollen tubes reached the ovules of the WT at 90–120 MAF. g and h few or zero pollen tubes reached the ovules of lssr1 lines at 90–120 MAF. i-k Retardation of pollen tube growth in lssr1 lines at 90–120 MAF. Bars = 100 μm. White arrows point to pollen tubes in a-c, f and g, and pollen tube tips in i-k. Red arrows point to pollen-tube-like dot lines on the stigma. l Quantification of ovaries with pollen tubes observed in the ovule at 90–120 MAF. Data are means ± SD from 3 replicates with ≥ 23 pistils per replicate, and “**” indicates a significant difference between the WT and lssr1 lines at P < 0.01 by the Student’s t-test