Fig. 6
From: LSSR1 facilitates seed setting rate by promoting fertilization in rice
lssr1 exhibits significant low seed setting rate. a Seed setting rate of lssr1 lines with mutation in the first targeted site. Data are means ± SD from ≥ 6 individuals with 5 main panicles per plant. b Seed setting rate of the subsequent lssr1 knockout individuals targeted in site2 and site3. TWT indicates the transgenic plant without mutation in LSSR1. Data are means + SD with 5 main panicles. c Partial image of the co-segregation analysis in segregated T2 populations. The genotypes were assessed by CAPS primer 38260 s1-F/R and restriction enzyme BseNI. −/− is one of a T2 individual from homozygous lssr1 line L1–5, and red arrows indicate T2 individuals with a low seed setting rate from heterozygous lssr1/+ line L1–3 when compared with the WT. d Reciprocal crosses between the WT and lssr1 mutant lines. Data are means ± SD of lg(seed setting rate(%) + 1) (for normalization) with ≥ 8 panicles. “**” indicates a significant difference at P < 0.01 by the Student’s t-test between the WT and L1 lines in a, between TWT and L2 or L3 individuals in b, and between the counterpart combinations in d