Identification and Characterization of LARGE EMBRYO, a New Gene Controlling Embryo Size in Rice (Oryza sativa L.)

Rice

Table 1 Segregation of F₂ seeds derived from the reciprocal crosses among HC and three giant embryo mutants

Cross combination	EL of F₁ seed (mm)	Number of different embryo type				Expected ratio	x ²	p-value
Cross combination	EL of F₁ seed (mm)	nor	le	ge	ge ^s	Expected ratio	x ²	p-value
HC/le	2.10^a	299	99	–	–	3:1	0	1
le/HC	2.10^a	260	100	–	–	3:1	1.34	0.25
HC/ge	2.16^a	348	–	134	–	3:1	1.87	0.17
ge/HC	2.16^a	269	–	83	–	3:1	0.31	0.58
HC/ge^s	2.12^a	246	–	–	76	3:1	0.27	0.61
ge^s/HC	2.12^a	265	–	–	84	3:1	0.12	0.73
le/ge	2.15^a	177	64	71	–	9:3:4	1.16	0.56
ge/le	2.15^a	145	48	63	–	9:3:4	0.02	0.99
le/ge^s	2.21^a	178	60	–	78	9:3:4	0.02	0.99
ge^s/le	2.21^a	160	51	–	63	9:3:4	0.67	0.72
ge^s/ge	3.12^b	–	–	296	117	3:1	2.27	0.13
ge/ge^s	3.12^b	–	–	106	45	3:1	1.61	0.2

The means followed by lowercase letters are significantly different (p < 0.05, ANOVA) according to Duncan test. EL embryo length, nor normal type