Fig. 4

Map based cloning of the LE gene. a Schematic diagram of mapping of LE locus. b Candidate ORFs in delimited candidate region. c LE gene structure and splicing pattern. Black lines, white solid boxes, and black solid boxes indicate intron, UTRs, and exons, respectively. The mutation position is marked with red arrow head. d LE protein structure and comparison of amino acid sequence of the frameshift region between the HC and le mutant. Asterisk represents the location of amino acid substitution caused by one base deletion in le mutant. Amino acids in the region of frameshift (fs) are indicated in red. Red background and black open boxes indicate amino acid sequences of RING C3HC4 domain and conserved residues in the domain