Fig. 2

Comparation of TH1 locus between KO and Non-KO lines. a Genomic structures at the TH1 locus in KO and Non-KO line. Arrows indicate the qRT-PCR primers for TH1. b The original sequencing chromatograms of the target site for TH1 in Non-KO and homozygous KO line, which were derived from a T₀ heterozygous KO plant. The protospacer adjacent motif (PAM) site is underlined, the “C” marked with an asterisk was the deleted in the KO plant. c Expression level of TH1 in KO and Non-KO line. Values are shown as means ± s.d. (n = 3). P values were calculated by the Student’s t-test