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Fig. 6 | Rice

Fig. 6

From: Reduction in sucrose contents by downregulation of fructose-1,6-bisphosphatase 2 causes tiller outgrowth cessation in rice mutants lacking glutamine synthetase1;2

Fig. 6

Isolation and analysis of OscFBP2 rice mutants. a Diagram of the insertion point of the retrotransposon Tos17 (triangle) in the OscFBP2 gene. Exons are indicated as boxed regions, lines represent introns, and open boxes correspond to 5′- and 3′-untranslated sequences. b-d Analyses of gene expression (b), cFBPase activity (c), and sucrose content (d) in the basal portions of the wild-type shoots (WT: black column) and those of two oscfbp2 mutants (oscfbp2-m1, gray column; oscfbp2-m2, open column). Seedlings were grown hydroponically in the presence of 1 mM NH4Cl until the fourth leaf stage. e, f Tiller number (e) and leaf stage (f) of wild-type plants (WT: black column) and two oscfbp2 mutants (oscfbp2-m1, gray column; oscfbp2-m2, open column) at 50 days after germination. Seedlings were grown hydroponically in the presence of 1 mM NH4Cl. Mean values plus the SE of five independent plants in (b-d) and four independent plants in (e, f) are indicated. Asterisks denote statistically significant differences between the WT and each oscfbp2 mutant (*, P < 0.05 by Student’s t-test)

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