Fig. 1

Overexpression of MKKK62 in ZH11 resulting in PHS. a, Germination phenotype of WT and OE panicles. At 30 DAH, panicles of OE1, OE2, OE3, and WT were sampled for germination tests. Seeds from OE lines germinated at 3 DAI. b, Germination percentage of seeds from OE panicles sampled at indicated times. Seeds were harvested at indicated times from OE plants for germination test. Germination percentage was calculated at 2 DAI. Values represent the mean ± SD of three biological replicates. c, Relative expression level of MKKK62 in seeds of MKKK62-overexpression (OE) lines at 23 DAH. At 23 DAH, gene transcript levels in seeds of OE1, OE2, OE3, and WT were analyzed by real-time PCR