Skip to main content
Fig. 1 | Rice

Fig. 1

From: Mapping the ‘early salinity response’ triggered proteome adaptation in contrasting rice genotypes using iTRAQ approach

Fig. 1

Flowchart depicting the experimental steps involved in the present study. Ten-day old rice seedlings of Pokkali and IR64 were subjected to salinity stress (200 mM NaCl) for a period of 2 h. To understand the mechanism of tolerance/sensitivity, shoot and root tissues were used for physio-chemical and proteome studies under control and salinity conditions. For the proteome studies, the protein extracts were trypsin digested and peptides were labeled with isobaric tags for iTRAQ reagents. For the peptide labeling, different iTRAQ labels (represented as 114 and 117; reporters with balance group 31 and 28 respectively) were used in this study. Labeled peptides were fractionated by strong cation exchange chromatography (SCX) and analyzed using LC-MS/MS (Liquid chromatography–mass spectrometry). POK represents Pokkali genotype and R1, R2 and R3 represent the replicates 1, 2 and 3 respectively

Back to article page